(W1166) Feasibility of 3D human induced pluripotent stem cell-derived osteoblast using Air-Liquid interface method for spinal fusion in a rat model; Preliminary report
Post Doc Catholic University of Korea Seoul, Seoul-t'ukpyolsi, Republic of Korea
Abstract: Spine fusion (SF) is one of the most common strategies for the treatment of various spine pathologies, such as degenerative disc diseases, trauma, tumor or infection. To achieve fusion of mobile vertebral segments, bone graft materials play an important role. However, harvesting autologous bone can cause morbidities at donor sites, and its amount is limited. The aim of this study was to investigate the efficacy of bone formation after transplantation of 3-Dimension iPSC derived osteoblast differentiation in Air-Liquid interface (3D iOB) in a spinal fusion animal model. Differentiation of 3D iOB using an Air-Liquid interface method in vitro. Spine fusion rat model was generated using 7-week-old adult male Sprague-Dawley rats. Those were allocated into four groups based on the graft materials: (1) SF only, (2) SF+Collagen Ⅰ without cells, (3) SF+demineralized bone matrix (DBM), and (4) SF+3D iOB. Transplantation of 3D iOB in the spinal fusion rat model improved bone volume and trabecular thickness at 8- and 12-weeks post transplantation. The results of this study suggest that iPSC-derived osteoblasts can be a powerful cell source for spinal fusion. In addition, the possibility of promoting bone formation using 3-Dimension iPSC derived osteoblast differentiation in Air-Liquid interface as well as its utility as a graft scaffold.
Funding Source: This work was supported by the National Research Foundation of Korea (NRF) and ministries including Science and ICT, Trade, Industry and Energy and Drug Safety (RS-2023-KH142779, RS-2019-NR040058).
