Abstract: The molecular features and multifaceted roles of platelets have been extensively studied in adult mammals, but little is known about platelets at other developmental stages, such as in embryos. In this study, we performed transcriptomic and proteomic profiling of platelets isolated from mouse embryos, revealing that while classic immune-regulatory and procoagulant features were reduced, development-supporting characteristics were particularly prominent in embryonic platelets compared to adult ones. Notably, embryonic platelets showed significantly stronger interactions with multiple cell types, including fibroblasts, and were markedly more effective in accelerating refractory wound repair. We identified a subpopulation of CD59a+ platelets that mirrored the effects of embryonic platelets. Furthermore, human induced pluripotent stem cell (hiPSC)-derived platelets exhibited molecular profiles similar to those of embryonic platelets. Our research underscores the unique multi-omics characteristics of embryonic and hiPSC-derived platelets, highlighting their superior capacity for tissue repair compared to adult platelets. These insights pave the way for advancing therapeutic applications by prioritizing the use of platelets from different developmental stages and sources tailored to specific medical needs.
