Abstract: The aim of this research is to isolate primary oral mesenchymal cell cultures (OMSC) from adult (DPSC) and deciduous tooth pulp (SHED), periodontal ligament (PDL), dental apical papilla (SCAP), gingiva (GMSC) and bone marrow (BMSC). Further more to characterize them for expression of specific mesenchymal stem cell markers and evaluate their ability to differentiate to different cell types.
The cells were characterized for expression of stem-cell markers (Nestin, Vimentin, CD146, CD44, CD49f) and markers associated with dental tissues (ALP, COL1A2, COL3A2, DSPP) using immunofluorescence. We made statistical analysis of the expression of the fluorescent markers with In Cell Anazyzer Wokstation 3.7.3. All cell cultures were subdued to osteogenic, adipogenic, chondrogenic and epithelial differentiation conditions. Differentiation was determined by Alizarin Red S, Oil Red O and Alcian Blue and expression of epithelial markers CK10 and p63.
We proved that OMSC express characteristic stem cell markers nestin, vimentin, CD44, CD49f, CD146. The statistical analysis revealed the different levels of expression in the studied stem cells cultures. We estimated, also the differences in the ability of OMSC to undergo osteogenic, adipogenic chondrogenic and epithelial differentiation compared with BMSC.
Revealing the phenotype of the OMSC and their differentiation readiness gives us invaluable information about the potential of these cells and will help us understand better the regenerative potential of OMSC, compared with BMC.
Funding Source: Bulgarian National Science Fund МП21 / 3.4 / BG-RRP-2.004-0004-C01
