Abstract: Adipose tissue engineering is an emerging field that repairs tissue defects from trauma or tumor resection and serves as filling materials in plastic surgery. Currently, in vitro 3D culture of adipose tissue overcomes the limitations of 2D models and complex animal studies, closely mimicking the physiological environment of real fat and applying on research of adipose-related diseases. The extracellular matrix (ECM) is crucial for adipose tissue, providing structural support and facilitating essential functions like cell adhesion, migration, and differentiation. However, existing studies often neglect the specific biochemical composition and mechanical properties of the extracellular matrix (ECM) that are essential for effective adipogenesis. By utilizing combinations of Collagen I (Col I), Collagen VI (Col VI), Fibronectin (FN), and Glycosaminoglycans (GAGs), we aim to fabricate dynamic ECM niches with controllable compositions of ECM components. Adipose-derived stem cells (ADSCs) sourced from autologous adipose tissue possess multipotent differentiation potential and secrete various growth factors. To evaluate scaffold functionality, we utilized ADSCs and induced adipogenic differentiation. Our findings indicated that the Col-GAG, Col VI, and combined culture groups effectively promoted adipogenesis. This study will demonstrate that our ECM-mimicking scaffolds can support adipogenesis and provide a foundation for future tissue regeneration research. By advancing the design of hydrogels that mimic native ECM, this work aims to significantly contribute to adipose tissue engineering and foster innovative solutions for tissue repair in clinical settings.
Funding Source: This work was supported by the Health@InnoHK program of the Innovation and Technology Commission of the Hong Kong SAR Government.
