(F1173) REGENERATION OF LARGE BONE DEFECTS USING MOUSE-DERIVED CALVARIAL OSTEOBLAST-LIKE CELLS AND THREE-DIMENSIONAL COTTON-LIKE POLYLACTIC ACID SCAFFOLD
Abstract: In dentistry, bone substitutes have often been used to regenerate alveolar bone. In recent years, new bone substitutes with three-dimensional structures have been developed, and only small bone defects can be completely healed. However, the healing of large bone defects where there is no surrounding bone remains difficult and bone tissue engineering approach combining scaffold materials and cells is thought to be necessary. Mesenchymal stem cells (MSCs) have been the main cell source for tissue engineering. Although MSCs have the ability to promote healing through their anti-inflammatory effects, there are few clinical reports that the cells themselves have a clear bone regenerative ability. Therefore, it is necessary to develop a new bone regeneration technique that combines cells with high bone-forming capacity with a scaffold that enables vertical bone formation. In this study, we developed a composite of mouse-derived calvarial osteoblast-like cells (MCOB) with bone regeneration ability and a three-dimensional cotton-like polylactic acid (3DPLA) scaffold. The MCOB-3DPLA composite demonstrated bone regeneration ability in mouse alveolar bone defect models, and nanoindentation analysis showed that the regenerated bone exhibited micromechanical strength similar to that of healthy bone. When titanium implants were placed in the regenerated bone, osseointegration with the implant surface was observed. Based on these results, it is believed that a new bone regeneration technology could be developed by isolating human cells using a method similar to MCOB.
