Phd Student Korea University College of Medicine Seoul, Republic of Korea
Abstract: An ideal neural organoid culture system should possess sufficient scalability and flexibility in modulating cellular diversity and developmental timing to better model human brain development. However, existing culture systems are often insufficient to achieve both scalability and flexibility at the same time. In this study, we developed a hydrogel scaffold-based culture system specifically designed to address these challenges. The interconnected pore network within the hydrogel facilitates efficient media perfusion, enhancing long-term viability and sustained culture. Through adjustment of seeding cell density, we observed tunable patterns in organoid quantity and size distribution, enabling scalable production. Furthermore, this system permits controlled temporal integration of biological or chemical components, creating a versatile platform for experimental modulation. We demonstrated the platform's capability to establish temporally controlled neural organoid networks and achieve multi-lineage integration within composite organoids. These findings highlight our hydrogel scaffold system's potential as a scalable and adaptable platform for investigating neural development, network formation, and cellular interactions within a controlled 3D environment.
Funding Source: Brain Korea 21 Plus Program For Biomedical Science
