Postdoctoral Fellow Guangzhou National Laboratory, China
Abstract: Understanding the developmental processes that lead to the formation of tissues and organs is a fundamental issue in developmental biology. In mammals, this process occurs after the embryo is implanted into the uterus, making observation and manipulation relatively difficult. Therefore, the sequence of developmental events from gastrulation formation to organogenesis remains to be fully understood and difficult to manipulate. The conventional mouse embryo culture system from E6.5 to E8.5 has certain applications in embryonic development research, but there are also some obvious drawbacks. Traditional static culture systems usually only provide a two-dimensional culture environment and cannot fully simulate the three-dimensional development process of embryos in the mother's body under natural physiological conditions. The oxygen and nutrients in the culture medium may not be evenly distributed in a static culture environment, resulting in uneven nutrition obtained by the embryo. And the embryos will deposit at the bottom of the culture bottle due to gravity, which may cause morphological irregularities or physical damage to the embryos. The ultimate result is usually a lower embryo development rate. Therefore, with the deepening of research, new cultivation methods, such as rotating culture systems, support embryos to have better in vitro developmental abilities.We further explored and developed the instrument structure and composition of the culture medium in the whole embryo in vitro rotation culture system, and found that our developed system can develop embryos from E7.5 stage to E11.5 stage at a rate of 100%. At the same time, we are also trying to combine the embryo rotation system with other systems modified in our laboratory to culture E11.5 to later stage embryos, and have made some progress.
