Graduate Student Tokyo University of Agriculture and Technology Fuchu-shi, Tokyo, Japan
Abstract: Feline mammary tumor (FMT) is known as a highly malignant and intractable disease. Due to the scarcity of appropriate experimental models, the availability of efficient treatment options is few. Previously, we had established the FMT organoid culture method derived from patient cats and evaluated its usefulness as a feline patient-derived culture model. In this study, FMT organoid was mainly classified with the “Basal” or “Luminal” type. We performed single-cell RNA sequencing (scRNA seq.) using two different FMT organoid lines to evaluate the genetic components in various organoid-derived cell phenotypes. The gene expression identified in the clusters was compared between basal and luminal organoids. They were classified into 13 clusters in basal or luminal-type organoids. In the basal cell cluster, expression of KRT5 and KRT17 (basal cell markers) and FABP5 (fatty acid metabolism marker) were increased, while in the luminal-type organoid, the expression of KRT19 and KRT8 (luminal cell markers) were upregulated. In KEGG analysis, the cell adhesion-related signals were activated in basal compared with luminal-type organoids. We for the first time demonstrated that the markers related to morphology in each FMT organoid were expressed, and there are differences in the pathway between basal and luminal organoids.
