Founder and Managing Director Cellenion SASU Cellenion SASU, France
Abstract: Complex three-dimensional (3D) in vitro cell models, including spheroids, tumoroids, and organoids, are becoming indispensable in various fields, aiding drug discovery and personalized medicine. However, transitioning from traditional monolayer cell cultures to 3D cell models poses significant challenges in sample handling, assay read-out, and overall assay reproducibility. This study presents an innovative workflow for high-throughput preparation of large quantities of spheroids in microcavity microplates, sorting and isolating homogeneous 3D models using spheroONE, and real-time live-cell analysis of drug response using the Incucyte® Live-Cell Analysis System. In this proof-of-concept, thousands of Human Embryonic Kidney (HEK293) spheroids were prepared in microcavity plates. After 3 days of culture, spheroids with diameters ranging from 190 to 260 µm were sorted and isolated as single spheroids per well in 96-well plates using spheroONE. Immediately following spheroid isolation, various concentrations of camptothecin were applied, and drug response was monitored over 7 days of culture through live-cell imaging and analysis using the Incucyte® Live-Cell Analysis System. Workflows combining spheroONE and Incucyte® live-cell imaging and analysis are adaptable to a variety of microphysiological systems (including spheroids, tumoroids, and organoids) and provide researchers with powerful solutions for accelerating toxicology and drug development studies.
