Innovation Showcases
.png)
The optimized protocols for expansion and differentiation of iPS cells on a small laboratory scale are subject to extreme degradation against target cell yield and its stability between when scaled up to full-scale production. It is desirable to develop methodology for equivalent performance in mass production with scale-up. In particular, cell death in the early phase of iPS expansion (after cell seeding to initiation of cell division) and in the early induction phase of differentiation (first step of induction from undifferentiated state) are considered to impact the yield and its batch-to-batch variability of the target differentiated cells. Here, I will present breakthrough strategies using novel reagents that improve yield and stability in mass cell production.