Innovation Showcases
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Although the potential of iPSCs to differentiate into various cell types has shown great application promise in regenerative medicine, variation in differentiation efficiency and low reproducibility significantly limit their practical application. Here, I will introduce a novel culture strategy for generating synchronous iPSC-derived differentiated cells using botulinum hemagglutinin (HA). This approach is not only a simple and efficient way to increase iPSC homogeneity, but also a synchronous differentiation method to produce target cells during multistep differentiation. HA may play an important role as a tool for culture stabilization because it can improve the robustness of the differentiation process of various differentiated cell types such as hepatocytes, pancreatic progenitors, cardiomyocytes, neural progenitors, and retinal pigment epithelial cells.