Innovation Showcases
Mesenchymal Stem/Stromal Cells (MSCs) have significant therapeutic potential but require large-scale manufacturing for clinical applications. Traditional 2D static culture methods are unsustainable at scale and require manual operations. Successful transition from 2D to 3D microcarrier-based Stirred Tank Reactor (STR) expansion is an important step toward fulfilling the commercial demand of clinical grade MSCs. In this study, we demonstrate how a small-scale static culture was transitioned to a suspension-based culture at 2L and then subsequently scaled up to 30L. Join this presentation to learn:
• Transition from 2D static culture to 3D suspension-based culture using a xeno-free media
• Successful scale-up of the expansion process to 30L scale using an STR
• Maintain consistent phenotypic characterization, immunosuppressive function, and karyotypic stability of cells across different scales