Ms Inha University Incheon/Inha University, Republic of Korea
Abstract: Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare genetic disorder characterized by the accumulation of the abnormal progerin protein, which leads to restricted cell proliferation and premature aging. This study investigated the effects of xeno-free media on the proliferative capacity of HGPS fibroblasts. Commercially available HGPS skin fibroblasts were cultured in conventional Eagle's Minimum Essential Medium (EMEM) and xeno-free media, and the resulting changes were analyzed. HGPS cells cultured in xeno-free media exhibited significant alterations, including decreased cell size, absence of cell stacking, increased cell number, and reduced population doubling time. Additionally, there was an increase in BrdU-positive cells, elevated levels of phosphorylated retinoblastoma protein (Rb), decreased expression of p21 and progerin, enhanced mitochondrial health, and reduced β-galactosidase-positive cells. In conclusion, xeno-free media demonstrates the potential to inhibit the senescence of HGPS fibroblasts. Further research is warranted to elucidate the specific mechanisms underlying these effects.
