(F1296) HETEROGENEITY OF MESENCHYMAL STEM CELL-DERIVED EXTRACELLULAR VESICLES: INFLUENCE OF CELL ORIGIN AND CULTURE CONDITIONS ON MOLECULAR CARGO AND SKIN REGENERATIVE POTENTIAL
Assistant Professor Chulabhorn International College of Medicine, Thammasat University Klong Luang, Pathum Thani, Thailand
Abstract: Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) display significant heterogeneity, influenced by their cellular origin and culture conditions. This study investigates the diversity of MSC-EVs derived from adipose tissue, umbilical cord, amniotic fluid, and corneal sources, analyzing their intravesical cargo and molecular profiles. Additionally, we examine the impact of hypoxia and normoxia culture conditions on EV composition and functionality. Using advanced molecular characterization techniques, we identify distinct signatures across MSC subtypes, highlighting protein, lipid, and RNA content variations. Functional assays in in vitro and ex vivo skin models further evaluate the regenerative potential of these EVs. Our findings underscore the critical role of MSC source and environmental factors in shaping EV therapeutic efficacy, offering insights for optimizing MSC-EV-based skin regeneration therapies.
Funding Source: Thailand Science Research and Innovation Fundamental Fund Fiscal Year 2025 Bioflirt Labs.Co.,ltd.
