Master Student Dankook University, Republic of Korea
Abstract: One of the stem cell therapies using mesenchymal stem cells (MSCs) has a great possibility for treating innumerable diseases because of their capacity to heal injured tissue/organ through the production of paracrine factors and immunomodulation. Even with high expectations, the elevated oxidative stress levels and low viability of transplanted cells in the injured tissue remain the biggest obstacles in the MSC-based cell therapy. To achieve the desired treatment efficiency, transplanted MSCs must be guaranteed to survive in a high oxidative stress environment. In this research, ROS-scavenging nanozyme is suggested to protect transplanted MSCs from the self-destruction of oxidative stress-mediated cells and improve the treatment effect. Prussian blue (PB) nanoparticles were impregnated as biocompatible ROS-scavenging nanozymes and incorporated into MSCs by endocytosis, which did not affect MSCs’ main characteristics and differentiation potential. Nanozyme impregnation not only greatly improved MSCs survival under high oxidative stress conditions but also strengthened paracrine effects, anti-inflammatory properties and presented deep therapeutic effects in hepatic ischemia-reperfusion damaged animal model. The results indicated that impregnating PB nanozymes into MSCs is a simple but efficient way to improve the potential of MSC-based cell therapy. Also, it could be a promising candidate for diseases associated with high oxidative stress and inflammation that need ROS-scavenging treatments.
