Abstract: It has been widely accepted that mammalian neural stem cells (NSCs) exist only in the central nervous system. Here, we report that peripheral NSCs (pNSCs) exist outside of the CNS and can be isolated—from mouse embryonic limb, postnatal lung, tail, dorsal root ganglia, and adult lung tissues. Derived pNSCs are distinct from neural crest stem cells, express multiple NSC-specific markers and exhibit cell morphology, self-renewing capacity, genome-wide transcriptional profile, epigenetic features, and differentiation ability similar to those of control brain NSCs. pNSCs are composed of Sox1+ cells and originate from neuroepithelial cells. Single-cell RNA sequencing identifies that pNSCs in situ have similar molecular features to NSCs in the brain. Furthermore, many pNSCs that migrate out of the neural tube can differentiate into mature neurons and limited glial cells during embryonic and postnatal development. Our finding of the existence of pNSCs provides previously unidentified insight into the mammalian nervous system development and presents an alternative potential strategy for neural regenerative therapy.
Funding Source: This project is supported by Health@InnoHK, Innovation Technology Commission, HKSAR
