Professor Seoul National University Hospital Seoul, Republic of Korea
Abstract: Mesenchymal Stem Cells (MSCs) are accepted as great cell source for regenerative medicine. Companies move to commercialize MSCs in clinical application. However, their efforts may be limited by the ability to expand their cell numbers in vitro with maintaining good quality of differential potentials and stemness. Previous studies from our group have shown reprogramming induction ability of shikimic acid from plant stem cell extracts through mannose receptor mediated mechanism. In this study, mannose bioisostere of the mannose receptor, enhance the rate of proliferation, self-renewal and anti-senescence of MSCs without loss of differentiation potential. Proliferation enhancement by quinic acid was mediated by Cyclin E. Mannose bioisostere induced Sox2, Nanog, Oct4 and Tert expression by binding to the mannose receptor and leading to MKK1/2/3/6, ERK1/2, P38 and CREB phosphorylation. We confirmed that mannose bioisostere inhibited ageing and enhanced regeneration of mouse heart in myocardial infarction model. These results indicate that quinic acid is an effective agent for expanding MSCs with delayed senescence.
Funding Source: National Research Foundation of Korea(NRF) (RS-2024-00359519)
