Assistant professor Medical University, Sofia Sofia, Bulgaria
Abstract: Bacteriophages are increasingly recognized as a viable alternative to antibiotics for combating bacterial infections. However, their biocompatibility with mammalian cells is a critical consideration for therapeutic applications. This study aimed to evaluate the cytotoxic effects of commercially obtained bacteriophages on stem cells from the apical papilla (SCAP), a key cell type in dental tissue regeneration.
SCAP cells were exposed to two concentrations of bacteriophage preparations (0.5 mL and 1 mL) in vitro, and cellular viability was assessed using the MTT assay. Results demonstrated that bacteriophage exposure at both concentrations resulted in minimal cytotoxic effects, with cell viability remaining above 90 at the tested doses. The findings suggest that the bacteriophages tested are generally biocompatible with SCAP cells at therapeutic concentrations.
This study highlights the importance of evaluating the safety of bacteriophages on mammalian cells and supports their potential use in clinical applications. Further research should explore the long-term effects of phage exposure and their interactions with other cell types in regenerative medicine.
