MS Peking University Third Hospital Beijing, Beijing, China (People's Republic)
Abstract: Cynomolgus monkey blastoids have shown similar morphology and lineage composition to primate blastocysts in previous studies. However, their application was limited by low efficiency and undefined cell clusters. Here, we present a novel and efficient protocol for generating cyBlastoids using 4CL naïve cyPSCs. These cyBlastoids exhibit morphology and cell lineage allocation similar to cynomolgus monkey blastocysts. Transcriptomic analysis confirms that the three corresponding cell lineages closely resemble those of natural blastocysts and other cynomolgus monkey blastoids. Furthermore, prolonged in vitro culture of cyBlastoids reveals typical morphology and cell lineage characteristics of post-implantation embryos. This stable and efficient protocol offers a robust model for primate embryo research.
